HPLC (High-Performance Liquid Chromatography) vials are used to store and inject samples into HPLC instruments. The vials come with caps and septa (or septums) to ensure airtight sealing and prevent contamination.
Key characteristics:
1. Material: Borosilicate glass or polypropylene
2. Volume: Typically 1-2 mL
3. Cap: Screw-top or crimp-top
4. Septa: Rubber or PTFE (polytetrafluoroethylene)
5. Septum thickness: 0.5-1.5 mm
Uses:1. Sample storage and injection for HPLC analysis
2. Chromatography and mass spectrometry applications
3. Pharmaceutical, biotech, and environmental testing
Handling and care:1. Handle with gloves to prevent contamination
2. Clean with methanol or acetonitrile before use
3. Ensure tight sealing with cap and septum
4. Store in a cool, dry place
Types of septa:1. Rubber septa: General-purpose, suitable for most HPLC applications
2. PTFE septa: Inert, suitable for sensitive or corrosive samples